https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51293 Wed 30 Aug 2023 13:27:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44982 Tridacna maxima) samples obtained from nine French Polynesian locations and tested a dual-index sequence library preparation method that pools and simultaneously sequences multiple Symbiodiniaceae gene amplicons per sample for in-depth biodiversity assessments. The rationale for this approach was to allow the metabarcoding of multiple genes without extra costs associated with additional single amplicon dual indexing and library preparations. Our results showed that the technique effectively recovered very similar proportions of sequence reads and dominant Symbiodiniaceae clades among the three pooled gene amplicons investigated per sample, and captured varying levels of phylogenetic resolution enabling a more comprehensive assessment of the diversity present. The pooled Symbiodiniaceae multi-gene metabarcoding approach described here is readily scalable, offering considerable analytical cost savings while providing sufficient phylogenetic information and sequence coverage.]]> Wed 26 Oct 2022 09:00:33 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54413 Tue 27 Feb 2024 14:00:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49452 Thu 18 May 2023 10:27:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48856 400 biosynthetic gene clusters that underlie the biosynthesis of antioxidant, antimicrobial, cytotoxic, and other secondary metabolites. Moreover, we uncovered genomic features—not previously described for coral-bacterium symbioses—potentially involved in host colonization and host-symbiont recognition, antiviral defense mechanisms, and/or integrated metabolic interactions, which we suggest as novel targets for the screening of coral probiotics. Our results highlight the importance of bacterial cultures to elucidate coral holobiont functioning and guide the selection of probiotic candidates to promote coral resilience and improve holistic and customized reef restoration and rehabilitation efforts.]]> Thu 13 Apr 2023 13:36:58 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46613 Cladocopium sp. (syn. clade C3) within the host coral Acropora aspera during exposure to thermal stress. Exposure to temperatures between 2 and 3°C below the bleaching threshold, equating to 2-degree heating weeks (DHWs), results in changes to the symbiont cell morphology and cell division rates. Once corals were exposed to 4 DHWs, over 90% of the symbiont cells showed signs of degradation. Although sub-bleaching thermal stress is not sufficient to trigger bleaching alerts at an ecological scale, this stressor substantially affects the coral symbiosis. It is therefore vital that we begin to quantify how sub-bleaching thermal stress affects the fitness of Symbiodiniacea populations, their coral hosts and subsequently reefs worldwide.]]> Mon 28 Nov 2022 10:36:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35467 Mon 12 Aug 2019 16:16:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41291 96%) (environmentally responsive community) of the species-specific microbiome were in fact not found in association with the majority of individuals of the species. Only 0.1% (~21 phylotypes) of the species-specific microbiome of each species was shared among all individuals of the species (core microbiome), equating to ~3.4% of the resident microbiome. We found taxonomic redundancy and consistent patterns of composition, structure, and taxonomic breadth across individual microbiomes from the three coral species. Our results demonstrate that the coral microbiome is structured at the individual level. Importance: We propose that the coral holobiont should be conceptualized as a diverse transient microbial community that is responsive to the surrounding environment and encompasses a simple, redundant, resident microbiome and a small conserved core microbiome. Most importantly, we show that the coral microbiome is comparable to the microbiomes of other organisms studied thus far. Accurately characterizing the coral-microbe interactions provides an important baseline from which the functional roles and the functional niches within which microbes reside can be deciphered.]]> Mon 01 Aug 2022 12:02:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45456 Fri 28 Oct 2022 14:23:02 AEDT ]]>